9/22/2023 0 Comments X particles 3.5 slow downLong term gene silencing can be achieved by standard clonal selection methodologies using the pLKO.1 plasmid followed by puromycin selection. For these proteins, siRNA may be diluted out before the cell can efficiently degrade the mRNA. For genes with slow turnover, or potentially slow turnover, utilizing shRNA for long term silencing is not just a convenience, it is the only choice. ![]() Long term, stable gene silencing distinguishes short hairpin RNA constructs (shRNA) from other RNAi tools. siRNA has been shown to be effective for short-term gene inhibition in certain transformed mammalian cell lines, while shRNA offers an opportunity to potently and stably silence gene expression. The introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology. Gene silencing and knockdown using RNA interference is becoming routine. Starting with Lentiviral Transduction Particles
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